Wissenschaftliche publikation


02.03.2021 14:59
Review report Corman-Drosten
October 26th 2020 to send us a copy of the peer review report. 9 However, it may be used to help the physicians differential diagnosis when he or she has to discriminate between different infections of the lung (Flu, Covid-19 and sars have very similar symptoms). The design errors described here are so severe that it is highly unlikely that specific amplification of sars-CoV-2 genetic material will occur using the protocol of the Corman-Drosten paper. To have a validated universal SOP is essential, because it facilitates data comparison within and between countries. Cantab, Former Research Manager, Germany Addendum Update.12.2020: Author Contribution. Das Methodenkapitel besteht aus folgenden Elementen: Rahmenbedingungen, Datengewinnung und Datenauswertung. For a confirmative diagnosis of a specific virus, at least 3 specific primer pairs must be applied to detect 3 virus-specific genes. Am J Biomed Sci Res 2020.ID.001312.pdf ; Archive: /i76Hu 16 Eurosurveillance paper evaluation / review process: rosurveillance.

The test cannot discriminate between the whole virus and viral fragments. MY: Proofreading the analyses and research. For determining the infectious viral load). Ihr wissenschaftlicher Verlag in Hamburg Ob Sie Ihre Promotion in Mnchen, Leipzig, Kln oder Hamburg abschlieen, ob wir Ihre Doktorarbeit, Habilitation, Festschrift oder eine andere wissenschaftliche Arbeit verlegen : Wenn Sie einen Verlag finden wollen, mit dem die Verffentlichung unkompliziert. 1, the focus here should be placed upon the two stated aims: a) development and b) deployment of a diagnostic test for use in public health laboratory settings. We find severe conflicts of interest for at least four authors, in addition to the fact that two of the authors of the Corman-Drosten paper (Christian Drosten and Chantal Reusken) are members of the editorial board of Eurosurveillance. BWK: Proofreading the analyses and research. Two primers (RdRp_sarsr_F and RdRp_sarsr_R) have unusual and very low GC-values of 28-31 for all possible variants of wobble bases, whereas primer E_Sarbeco_F has a GC-value.6 (Table 3 and second panel of Table 3).

Im Gegensatz zu empirischen Arbeiten erfolgt die Methodenbeschreibung in Literaturarbeiten nicht so detailliert. Zuschsse fr Autorinnen und Autoren, im Jahr 2021 schreiben wir Ihnen einen Frderbetrag von bis zu 500 Euro aus unserem. To have a validated universal SOP is essential, because it enables the comparison of data within and between countries. Of course, one could perform 45 PCR cycles, as recommended in the Corman-Drosten WHO-protocol (Figure 4 but then you also have to define a reasonable Ct-value (which should not exceed 30). And what is their negative control? Ulrike Kmmerer her team. JB: Proofreading the analyses and research.

How many PCR cycles to run? But an analytical result with a Ct value of 45 is scientifically and diagnostically absolutely meaningless (a reasonable Ct-value should not exceed 30). At high primer concentrations this is enough to create inaccuracies. Good laboratory practice, and 3) parameters examined against relevant scientific literature covering the field. KMcK: Conducted  the analyses and research, conceptualized the manuscript. Why did the authors assume a challenge for public health laboratories while there was no substantial evidence at that time to indicate that the outbreak was more widespread than initially thought?

It is very important to specify all primer parameters unequivocally. Die wichtigen Inhalte werden stichwortartig zusammengestellt (Tabelle oder Spiegelstrichen). Aus Wikipedia, der freien Enzyklopdie, zur Navigation springen, zur Suche springen. A difference of 10 C with respect to the annealing temperature Tm for primer pair1 (RdRp_sarsr_F and RdRp_sarsr_R) also makes the test unsuitable as a specific diagnostic tool to identify the sars-CoV-2 virus. This means for a target-sequence to be recognized we have to choose a temperature which is as close as possible to the actual annealing-temperature (best practise-value) for the primer not to detach again, while at the same time specifically selecting the target sequence.

Die Literatur wird objektiv ausgewertet und zwar nach bestimmten Kriterien. Gnstige Verffentlichung bei hervorragenden Leistungen, der, wissenschaftsverlag. . The PCR for this N gene was not further validated nor recommended as a test gene by the Drosten-Corman paper, because of being not so sensitive with the sars-CoV original probe. What is their positive control? FF: Proofreading the analyses and research. Welche Schritte werden bei der Beweisfhrung unternommen.

The reverse primer hits Pantoea as well but not in the same region (Figure 3). Jan Bonte (GJ Consultant Neurologist, The Netherlands 20). Authors as the editors A final point is one of major concern. Four negative samples initially tested positive, then were negative upon retesting. Die Methode in den empirischen Arbeiten richtet sich laut Karmasin, Matthias/ Ribing, Rainer (2006, 110)2 auf das Ziel und die Fragestellung der Untersuchung. As indicated in table 3, three of the primers described in the Corman-Drosten paper are not within the normal range for GC-content. U.a.7 (2008, 136) finden sich die Ausfhrungen, dass die Einleitung einer wissenschaftlichen Arbeit die Funktion hat, unter anderem die Vorgehensweise der Arbeit zu beschreiben.

The published RT-qPCR protocol for detection and diagnostics of 2019-nCoV and the manuscript suffer from numerous technical and scientific errors, including insufficient primer design, a problematic and insufficient RT-qPCR protocol, and the absence of an accurate test validation. Erste Auflage 2003 9 Ebd. Der Datenbestand umfasst rund 1900 Publikationen. These six unspecified positions could easily result in the design of several different alternative primer sequences which do not relate to sars-CoV-2 (2 distinct RdRp_sarsr_F primers 8 distinct RdRp_sars_P1 probes 4 distinct RdRp_sarsr_R). (2012, 106)11 um den Gang der Untersuchung. Metaanalysen und berblicksarbeiten review-Artikel ).

Considering the scientific and methodological blemishes presented here, we are confident that the editorial board of Eurosurveillance has no other choice but to retract the publication. The Corman-Drosten paper further identifies a third gene which, according to the WHO protocol, was not further validated and deemed unnecessary: Of note, the N gene assay also performed well but was not subjected to intensive further validation because it was slightly less sensitive. Apparently the WHO recommends these primers. Auch Kruse 2010, 90)13. Kontaktaufnahme, information der Befragten ber das Forschungsprojekt. Laboratory results should always be interpreted in the context of the clinical presentation of the patient, and appropriate site, quality, and timing of specimen collection are required for reliable test results. The number of amplification cycles It should be noted that there is no mention anywhere in the Corman-Drosten paper of a test being positive or negative, or indeed what defines a positive or negative result. Anschlieend werden Kriterien zur Auswahl von einer Stichprobe oder Probanden angefhrt. Additional Proof-Readers: Saji N Hameed, Environmental Informatics, University of Aizu, Tsuruga, Ikki-machi, Aizuwakamatsu-shi, Fukushima, Japan Howard.

Vielen Dank fr die gute und klare Kommunikation, die mir sehr geholfen hat. Typically a journal will only publish an article once the editors are satisfied that the authors have addressed referees concerns and that the data presented supports the conclusions drawn in the paper. Thomas Binder, Internist and Cardiologist (FMH Switzerland 11) Prof. Our conclusion is supported by the fact that a tremendous number of very serious design flaws were found by us, which make the PCR test completely unsuitable as a diagnostic tool to identify the sars-CoV-2 virus. Campus Concept 5 esselborn-krumbiegel, helga (2010 Richtig wissenschaftlich schreiben. Negative samples that test positive. Second Panel shows a table-listing of all Primer-blast best practices values for all primers and probes used in the Corman-Drosten paper by Prof.

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